Vy Tran
Analyzing The Impact Of TBaP Against Lepidium Sativum
Summary
In conclusion, the effect of Tetrahydrobenzo[a]pyrene-7-ol (TBaP) has a high effect on the growth after overexposure. Although it results in a inconclusive result against the Biuret Protein Test; which will be proved by the inconclusive results given. Although the 0.3mg concentration had the highest amount of proteins (higher than the control), not enough information and data was collected to make a final conclusion against the amount of proteins. The reason why this experiment is important, is because when looking at the effect of the proteins and growth speed and length of lepidium sativum, it has a correlation with Polycyclic Aromatic Hydrocarbons. Additionally, the chemical used within this project is a confirmed carcinogen that affects the protein health, further having exposure towards a confirmed carcinogen after consumption; only if the vegetation is exposed to Polycyclic Aromatic Hydrocarbons. Is it also noteworthy that not all data collected is reliable and accurate; although, allowing for data that was resulted to be taken into consideration when looking at areas that is heavily polluted with different Polycyclic Aromatic Hydrocarbons. Reasonably, the data concluded will need further results to have a confirmed conclusion, although the effect of Polycyclic Aromatic Hydrocarbons does have a negative effect on the growth. To conclude, the effect of Polycyclic Aromatic Hydrocarbons (specifically the mutant of Benzo(a)pyrene) directly affects vegetation health, further exposing humans to more carcinogens.
Results
Trial 1
​When looking at trial 1, it is visible to see that the 0.3mg concentration had the highest amount of growth; this is further supported when looking at the final growth for all other concentration levels. Exceeding in a growth of 1.4cm higher than the control concentration. Not only is it shown in the table, but also the trendline as it has a higher amount, having a positive slope.
Trial 2
​When observing Trial 2, it is shown that again, the 0.3mg concentration has the highest amount of growth; although, the 0.1mg concentration and the control concentration died by day 5, it still results in 2.8cm higher in growth. This can also be compared when looking at the trendline graph, which has a negative slope regarding the 0.1mg concentration and control, further showing that the data for 0.1mg and control as unreliable and possibly inaccurate.
Trial 3
​Comparing Trial 3 to Trial 2, what can be seen is that the growth of lepidium sativum for concentration levels 0.2mg and 0.3mg died by day 11, similar to the death of the 0.1mg concentration and control in Trial 2. Although when looking at the trendline of the graph, The only concentration level that has a negative slope is the 0.3mg concentration, this is highly likely due to the sudden death of the concentration after having a higher growth rate.